Fig. 2.

Recombinantly expressed LDN and LDNF are cross-reactive with schistosome antigens. (A and B) Cells were fixed and stained with pooled antisera from naïve, 8- or 20-week low-dose S. mansoni-infected mice on flow cytometry. Bound IgM (left) or IgG (right) was detected with Alexa-488 or Alexa-633-conjugated secondary antibodies. White bars, Lec8 cells; gray bars, L8-GT cells; black bars, L8-GTFT cells. (A) Mean ± SEM of the geoMFI fold change of 20-week infected serum over naïve serum for three experiments is shown and one-way ANOVA with Tukey's multiple comparisons test was used. (B) Mean ± SD of the geoMFI fold change of 8- and 20-week infected serum over naïve serum for two independent experiments with kifunensine- (hashed bars) or mock-treated cells is shown and two-way ANOVA with Tukey's test used to compare the effect of kifunensine treatment independent of the serum used. (C) Cell lysates were western blotted with infected antisera, using parasite life stages as a positive control, and detected with HRP-conjugated anti-mouse IgG at 1:5000. Asterisk denotes non-specific bands that appear with secondary antibody and substrate only when SEA and adult worm lysates are stained. Kif, kifunensine; stds, molecular weight standards; Cerc., S. mansoni cercarial lysate; Som., schistosomula lysate; Adult, adult worm lysate; SEA, soluble egg antigen; Exp., exposure time; ns P ≥ 0.05; *P = 0.01–0.05; **P = 0.001–0.01; ***P = 0.0001–0.001; ****P < 0.0001.