Fig. 6.

L8-GTFT cell antisera binds to schistosome glycoproteins. (A) Binding to cell N-glycans was confirmed by western blotting of pre-bleed and week 20 L8-GTFT (FT) antisera against Lec8 and L8-GTFT (FT) cell lysate, mock or PNGaseF-treated to remove N-glycans. (B) Pre-bleed sera and antisera from individual L8-GTFT-immunized mice that responded highly in flow cytometry experiments were used to stain parasite life stage lysates, with infected mouse sera shown as a positive control in some experiments. (C) Low-exposure stains of SEA with anti-LDNF and infected sera are shown for comparison with the molecular weights stained by immune sera in (B). Panes separated by a line were run in the same experiment and membranes were cut to allow for incubation with naïve, immune or infected sera. Membranes separated by space were run on different days under similar conditions. SEA, soluble egg antigen; Cerc., cercarial lysate (B). Blots were detected with HRP-labeled goat anti-mouse IgG or IgM.