Lectin and monoclonal antibody binding specificities on the defined schistosome-type glycan array. (A) After incubation with the glycan microarray slides, bound biotinylated lectins (0.1 µg/mL) were detected by cyanine5-streptavidin. Only the lower concentration of each printed glycopeptide (even numbers) are shown. PHA-L, Phaseolus vulgaris agglutinin L; PHA-E, Phaseolus vulgaris agglutinin E; PNA, peanut agglutinin; HPA, Helix pomatia agglutinin; WFA, Wisteria floribunda agglutinin; WGA, wheat germ agglutinin; LTL, Lotus tetragonolobus lectin; AAL, Aleuria aurantia lectin; SNA, Sambucus nigra agglutinin; RCA I, Ricinus communis agglutinin I; ConA, concanavalin A; UEA I, Ulex europaeus I; MAL, Maackia amurensis lectin. (B) Binding of monoclonal antibody to LDN measured by Alexa488-labeled goat anti-mouse IgM secondary antibody. ID numbers on x-axes correspond to glycopeptides listed in Table I. RFU, relative fluorescence units.