Table 2.
Kinetic analysis of germination of wild-type and mutant gerD spores with l-valine
| gerD strains | Relative rates (min−1)a | C50 (mM)b | Vmax (min−1) |
|---|---|---|---|
| 1-180 | 100 | 2.2 ± 0.2 | 186 ± 5 |
| 1-185 (B. subtilis) | 113 | 2.4 ± 0.5 | 230 ± 17 |
| ΔgerD | 15 | 4.2 ± 1.2 | 38 ± 4 |
| Helix truncation | |||
| 1-28, 55-18 (ΔH01-02)c | 13 | 5.1 ± 1.5 | 29 ± 4 |
| 1-163 (ΔH8) | 80 | 2.9 ± 0.3 | 85 ± 3 |
| 1-146 (ΔH7-8) | 26 | 4.4 ± 1.3 | 61 ± 7 |
| 1-133 (ΔH6-8) | 7 | 6.2 ± 1.2 | 26 ± 8 |
| 1-120 (ΔH5-8) | 14 | 3.9 ± 1.2 | 56 ± 6 |
| Helix disruption | |||
| M36P (H01) | 9 | 4.4 ± 1.5 | 18 ± 4 |
| F86P (H2) | 11 | 6.2 ± 1.7 | 40 ± 5 |
| M111P (H4) | 50 | 3.0 ± 0.6 | 104 ± 7 |
| M138P (H6) | 9 | 4.0 ± 1.3 | 20 ± 4 |
| M171P (H8) | 14 | 5.1 ± 1.5 | 29 ± 4 |
| Conservation | |||
| F96A (H3) | 4 | 7.4 ± 2.0 | 16 ± 2 |
| D118A (between H4 and H5) | 10 | 4.0 ± 0.4 | 27 ± 1 |
| Q122A (H5) | 7 | 9.7 ± 1.7 | 18 ± 2 |
| R148A (H7) | 7 | 7.5 ± 2.4 | 20 ± 3 |
a
The initial rate of the germination of the GerD1-180 spores was set at 100.
b
C50, germinant concentration that stimulates a half-maximal initial rate of germination.
c
Values in parentheses indicate the location of the mutation.