Table 1.
Conditional loss of En1/2 results in foliation defects caused by perturbations in the formation of specific fissures in the vermis and hemispheres. In the vermis, alterations of anterior fissures affect lobules I-V, and disruptions in posterior fissures affect primarily lobule VIII. Anterior fissure defects include an missing or shortened primary (m-pr and s-pr, respectively) and a shortened precentral fissure (s-prc), whereas posterior fissure defects include a deeper prepyramidal (d-ppy) an absent or posteriorly shifted secondary fissure (m-sec and ps-sec, respectively). In the hemispheres, loss of En1/2 can result in the missing of the ansoparamedian fissure (m-a). Genotypes colored blue represent conditional mutants with one functional allele of En1 and genotypes colored red represent conditional mutants with one functional allele of En2.
| m-pr | s-pr | m-prc | s-prc | m-sec | ps-sec | d-ppy | m-a | |
|---|---|---|---|---|---|---|---|---|
| NP-Cre; En2fx/− | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 4/4 | 4/4 | 4/4 |
| RLd-Cre; VZd-Cre; En2fx/− | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 |
| VZd-Cre; En2fx/− | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 |
| RLd-Cre; En2fx/− | 0/3 | 0/3 | 0/2 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| NP-Cre; En2fxlacZ/fxlacZ | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 |
| RLd-Cre; VZd-Cre; En2fxlacZ/fxlacZ | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| VZd-Cre; En2fxlacZ/fxlacZ | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 4/4 | 4/4 | 4/4 |
| RLd-Cre; En2fxlacZ/fxlacZ | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 3/5 | 3/5 | 5/5 |
| NP-Cre; En1fx/−; En2fx/− | - | - | - | - | - | - | - | - |
| NP-Cre; En1fx/+; En2fx/− | 3/3 | 0/3 | 0/3 | 2/3 | 3/3 | 0/3 | 3/3 | 3/3 |
| NP-Cre; En1fx/−; En2fx/+ | 0/4 | 0/4 | 3/4 | 1/4 | 0/4 | 4/4 | 4/4 | 0/4 |
| RLdCre; VZd-Cre; En1fx/−; En2fx/− | 2/5 | 3/5 | 2/5 | 3/5 | 5/5 | 0/5 | 5/5 | 0/5 |
| RLd-Cre; VZd-Cre; En1fx/+; En2fx/− | 0/4 | 0/4 | 1/4 | 0/4 | 0/4 | 4/4 | 4/4 | 0/4 |
| RLd-Cre; VZd-Cre; En1fx/−; En2fx/+ | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 3/3 | 3/3 | 0/3 |
| VZd-Cre; En1fx/−; En2fx/− | 0/5 | 3/5 | 0/5 | 1/5 | 2/5 | 3/5 | 5/5 | 0/5 |
| VZd-Cre; En1fx/+; En2fx/− | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 2/3 | 2/3 | 0/3 |
| VZd-Cre; En1fx/−; En2fx/+ | 0/5 | 0/5 | 0/3 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| RLd-Cre; En1fx/−; En2fx/− | 0/4 | 2/4 | 2/4 | 0/4 | 0/4 | 2/4 | 0/4 | 0/4 |
| RLd-Cre; En1fx+; En2fx/− | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | 0/3 | 0/3 |
| RLd-Cre; En1fx/−; En2fx/+ | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| RLd-Cre; VZd-Cre; En1fx/fx; En2 fx/fx | 1/6 | 5/6 | 2/6 | 1/6 | 6/6 | 0/6 | 6/6 | 0/6 |
| VZd-Cre; En1fx/fx; En2fx/fx | 0/8 | 5/8 | 1/8 | 0/8 | 3/8 | 5/8 | 8/8 | 0/8 |
| RLd-Cre; En1fx/fx; En2fx/fx | 0/6 | 4/6 | 2/6 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 |