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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: Cell Rep. 2013 Sep 26;5(1):3–12. doi: 10.1016/j.celrep.2013.09.003

Figure 1. Hotair knockout causes homeotic transformations.

Figure 1

(A) Schematic of Hotair conditional knockout (cKO) allele. Arrowhead, Loxp site.

(B) qRT-PCR confirms loss of Hotair expression in KO TTF.

(C) Micro-CT scans showing “L6a→S1” homeotic transformation of the lumbar vertebrae, which resulted in losing the 6th lumbar and having structurally deformed 1st sacral vertebrae (arrow) in Hotair KO mice. L, lumbar vertebrae; S, sacral vertebrae.

(D) Alizarin Red-Alcian Blue staining showing the deformed wrist bones in KO mice. Digits (II-V), carpal elements (1, 2, 3, 4/5), central element (c), radiale (r), ulnare (u), radius (ra) and ulna (ul). Note the fusion of carpal elements c-3, and 1-2-c (circled area), and missing radius (asterisk) in KOs. 4/5 are always naturally fused in WT wrist. (Scale bar: 0.5mm)

(E) Summary of skeletal abnormalities in Hotair KO mice. Phenotype penetrance, number (n) of animals examined, and p-values (Fisher’s exact test) are indicated.

See also Figure S1.