Figure 2. Characterization of MBs using Coulter Counter and Flow Cytometry.
(A) MB concentration was measured on Coulter Counter and flow cytometry. All types of MB preparations resulted in similar concentrations: have the same order of magnitude of about 109 MB/mL, as measured on both instruments. (B) Stability of MB concentrations post-activation. Relative MB concentration was measured on Coulter Counter 0, 5, and 10 minutes post-activation. These were normalized to the initial MB concentration (0 minute). Neutral MBs had similarly sustained MB concentration, while RC5K showed less stability. (C) Size profiles of various MBs evaluated by Coulter Counter III. (D) Size distributions of various MBs. Size histograms obtained from flow cytometry revealed that the peak MB diameters are similar for three MBs. Error bars indicate STD (n=3–5).