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. Author manuscript; available in PMC: 2015 May 28.
Published in final edited form as: J Control Release. 2014 Mar 18;182:111–120. doi: 10.1016/j.jconrel.2014.03.002

Figure 3. DNA loading on MBs after incubation.

Figure 3

(A) MB surface charge is measured using Zetasizer. Baseline zeta potential for Definity, RN18, and RC5k MBs were −4.04 mV, −1.77 mV, and +6.39 mV, respectively. Neutral MBs Definity and RN18 displayed little change in surface charge after DNA incubation (-2.72 ΔmV, and −0.72 ΔmV, respectively), while the cationic RC5k showed a large decrease in surface charge (-46.08 ΔmV) as the cationic lipid shell binds negatively-charged DNA. (B) After incubation with Cy5™ labeled DNA, Cy5™-positive MBs are detected in flow cytometry. RC5k showed increased DNAbinding capacities with longer incubation periods as indicated by higher proportion (93.4% vs. 51.5%) and MFI (273 vs. 59) of Cy5™-positive MBs compared to RN18. (C) Post-binding MFI of cationic and neutral MBs was categorized by MB size. This shows that bigger cationic MBs have higher MFIs with longer incubation time; whereas, MFIs of neutral MBs remained the same. (D) Post-binding Cy5™ –positive proportions of cationic and neutral MBs as separated by MB size. The proportion of Cy5™ positive population post-DNA incubation was greater in cationic MBs (>85%) but remained the same for neutral MBs (~65%). Error bars indicate STD (n=3). MFI, mean fluorescent intensity.

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