Figure 4.
(a) Binding/uptake of BLM monosaccharide–Cy5** and BLM monosaccharide–Cy5** trimer conjugates in A498 and A549 cell lines. Cells were treated with 25 μM BLM monosaccharide–Cy5** conjugate or BLM monosaccharide–Cy5** trimer at 37 °C for 1 h, washed and fixed. The nuclei were stained with DAPI. Fluorescence imaging was conducted with a 3 s exposure time. (b) Quantification of the binding/uptake of the BLM monosaccharide–Cy5** conjugate and the BLM monosaccharide–Cy5** trimer in six cancer cells. The cells were treated with 25 μM dye conjugates and irradiated for 3 s prior to being imaged.