Figure 4.

(A) Isothermal titration binding isotherm or the titration of Tel-22ss with UP1. Experiments were performed in Tris buffer [0.02 M Tris-HCl (pH 8.0) and 0.1 M KCl] at 25 °C. (B) Integration of the raw data to provide the binding enthalpy. The solid lines drawn through the data points represent best fits of a nonlinear least-squares model from which thermodynamic binding parameters can be estimated as described in the footnotes of Table 1.