. 2014 May 5;53(20):3327–3335. doi: 10.1021/bi500046t

Table 1. Binding Affinities of Hydroimidazolones and Hydroimidazolone-Containing Peptides for Wild-Type and Mutant V Domains.

molecule	V domain K_d (nM)^a	Q100A V domain K_d (nM)^a	R98A V domain K_d (nM)^a
MG-H1	40 ± 5	>10000	>10000
MG-H2	44 ± 5	–	–
MG-H3	31 ± 5	–	–
G-H1	43 ± 7	–	–
LSQ(MG-H1)FPKAE	35 ± 4	–	–
ALT(MG-H1)EYTG	44 ± 6	–	–

The dissociation constant was obtained by fitting fluoresceence titration data with a single-site binding isotherm.