Representative images of A6 cells used to calculate lipid-mediated transfection efficiencies. Cells transfected with pEGFP-N3 (A), pmCherry-N1 (E), pmCherry-N1 BK β1(I), and EYFP-tub (M) were counterstained with Hoechst (B, F, J, N) to calculate efficiency. Fluorescent merges (C, G, K, O) and DIC images (D, H, L, P) show that fluorescent labels localize to cells as predicted. Scale bar=50 μm.