Figure 4.

Embryos with ectopic PGCs in different treatment groups. In tank water, ICI and MPP treatment induced ectopic PGC distribution in 14% and 10% of the embryos, respectively. PHTPP, esr2a-MO, and esr2b-MO treatment did not disrupt PGC distribution. An increased percentage of fish with ectopic PGCs at 24 hpf was observed after exposure to EE2 compared with exposure to tank water alone (i.e., without EE2). Approximately 37% and 40% of the embryos displayed ectopic PGCs after exposure to ICI and MPP, respectively. Fewer embryos showed ectopic PGCs expression after exposure to PHTPP, and esr2a expression was reduced by approximately 13% and 11%, respectively. Inhibition of esr2b expression did not affect PGC distribution.