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. 2014 Mar 17;3(3):e94. doi: 10.1038/oncsis.2014.6

Figure 1.

Figure 1

Proteomic analysis of colorectal cancer cell lines revealed that lamin B2 is downregulated in CIN cell lines. (a) Proteomic analysis of CIN and MIN cell lines using agarose 2D-DIGE. Nuclear extracts prepared from the cell lines were labeled with Cy3 (MIN) and Cy5 (CIN), and separated with two-dimensional electrophoresis. Increased and decreased protein spots in CIN nuclei are displayed as red (Cy5) and green (Cy3), respectively. Yellow arrow shows the protein spot corresponding to lamin B2. (b) Western blot analysis of nuclear extracts (10 μg) from CIN and MIN cell lines using antibodies to lamin B2, B1 and lamin A/C. (c) Immunostaining of CIN and MIN cell lines using anti-lamin B2 antibody and DAPI for DNA. Images show interphase cells. Scale bars, 10 μm.