Figure 1.

Neutralizing effect of anti-S100P mAbs in S100P-induced proliferation. (a) Increased time-dependent proliferation induced by S100P protein (100 nM) in BxPC3 cell line. (b) Dose-dependent response of S100P in BxPC3 proliferation. Cells were treated with S100P (0.1–0.5–1 μM) for 48 h. (c) Neutralizing activity of mAbs (3F8, 2H8, 1A5 ad 3E3) at 500 nM on BxPC3 proliferation assay. Cromolyn (0.5 and 100 μM) was used as a referenced blocking product of the extracellular activity of S100P. Before stimulation, antibodies and Cromolyn were incubated with S100P (100 nM) for 2 h at 37 °C. Each data point was normalized to the basal proliferation of the cells without S100P, which represents 100% proliferation. Bars show the mean±s.e.m. of at least three independent experiments. ns P>0.05, **P<0.01, ***P<0.001.