Figure 4.
Effect of 2H8 mAb in tumor growth using a subcutaneous BxPC3 xenograft model. Female, athymic, nude mice were inoculated subcutaneously with 4 × 106 cells in 0.1 ml culture medium without supplements, into the right upper flank of mice. When tumors reached 65–160 mm3, treatment was initiated. Treatment groups had five animals. PBS (vehicle), 2H8 (25 mg/kg) and Cromolyn (5 mg/kg) was given three times a week (1010100) by intratumoral route (20 μl). Final formulation buffer was given as vehicle control. Graph of relative tumor volume shows the activity of the 2H8 and Cromolyn compared with the control group.