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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: Cancer Res. 2013 Jan 28;73(7):2368–2378. doi: 10.1158/0008-5472.CAN-12-3424

Figure 5. ST6Gal-I expression correlated with cancer stem cell enrichment.

Figure 5

(A) Colon carcinoma cells, HD3.par and HD3.sh, were assayed for ALDH1 activity (Aldefluor) by flow cytometry. Enrichment of ALDH1 staining was significantly higher in HD3.par as compared to HD3.sh in three independent runs. (B) Representative dot plot (run #1, 5A) showing ALDH1 staining. (C) Aldefluor and SNA-TRITC double-labeling shows knockdown decreases α2–6 surface sialylation along with stem cell enrichment. (D) Double labeling for stem cell enrichment of HD3.par and HD3.sh cells with ALDH1 and CD133 by flow cytometry revealed that knockdown of ST6Gal-I lead to significantly decreased enrichment in three independent runs. (E) Immunoblot of HD3.par and HD3.sh cells showed that shRNA transduction reduced ST6Gal-I expression. Densitometry completed by normalizing to respective β-actin and then comparing HD3.sh to HD3.par. *=P <0.001.