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. Author manuscript; available in PMC: 2014 May 29.
Published in final edited form as: Cancer Res. 2013 Jan 28;73(7):2368–2378. doi: 10.1158/0008-5472.CAN-12-3424

Figure 6.

Figure 6

(A) ALDH1 activity was assayed by flow cytometry in colon carcinoma cell line SW948. SW948.CPT chemoresistant line had significant enrichment for ALDH1 staining in three independent runs as compared to SW948.par. (B) Representative dot plot of ALDH1 staining 28 (run #1, 6A). (C) Double-labeling of SW948.par and SW948.CPT with ALDH1 and CD133 showed significant increase in stem cell markers in the chemoresistant line (SW948.CPT) in three independent runs. (D) Immunoblot of SW948.par and SW948.CPT shows ST6Gal-I expression was upregulated in the SW948.CPT line. Densitometry completed by normalizing to respective β-actin and then comparing SW948.CPT to SW948.par. (E) Double-labeling with Aldefluor and SNA-TRITC demonstrates chemoresistant line has increased stem cell enrichment as well as increased surface α2–6 sialylation. *=P <0.001.