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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Mol Cell. 2013 Jun 27;51(2):249–264. doi: 10.1016/j.molcel.2013.05.026

Figure 2. The ability of post-termination ribosomes with a vacant P-site to rebind tRNAs.

Figure 2

(A–C) Toe-printing analysis of ribosomal complexes obtained by treating pre-TCs formed on MVHC-STOP mRNA with eRF1, eRF1/eRF3 or puromycin in the presence/absence of (A, B) tRNAiMet and (C) tRNAHis or tRNALys at the indicated free [Mg2+]. Red arrows show the positions of ribosomal complexes arrested at AUG (Met), CAC (His) or AAA (Lys) codons.