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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Mol Cell. 2013 Jun 27;51(2):249–264. doi: 10.1016/j.molcel.2013.05.026

Figure 3. eEF2 destabilizes post-TCs and promotes ribosome migration.

Figure 3

(A–E) Toe-printing analysis of ribosomal complexes obtained by treating pre-TCs formed on MVHL-STOP, MVHC-STOP, MVKC-STOP or MVHL-STOP/C-less mRNAs with combinations of eRF1, eRF3, puromycin, eRF1(AGQ) and eEF2 at the indicated free [Mg2+]. Black arrows show the positions of pre- and post-TCs. Red arrows indicate the positions of migrated post-termination ribosomes. Upstream codons that are identical to the last codon of the encoded tetrapeptide are shown in red.