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. 2014 Mar 24;6:plu012. doi: 10.1093/aobpla/plu012

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Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Results of pairwise variation analysis (upper panel) and average expression stability calculations (lower panel) undertaken using GeNorm from the quantitative PCR of alfalfa gene transcripts using primers designed for eight potential reference genes (Phos, phosphoprotein phosphatase type 2A; His, histone H3; Cal, calmodulin; ADP, ADP-ribosylation factor; EIF, elongation initiation factor; GhyP, glyceraldehyde-3-phosphate dehydrogenase; GTP, GTP-binding protein; and Tum, translationally controlled tumour protein). The broken horizontal line on the upper panel represents the 0.15 suggested cutoff for determining the optimum number of reference genes required for normalization.