Figure 2. Identifying transcripts that change in abundance following SHP2 depletion or MEK1/2 inhibition in pellet cultures.

A: Immunodetection of SHP2, phospho-ERK1/2, total ERK1/2, and phospho-AKT in chondrocyte pellet cultures. Top: pellets from Ptpn11^fl/– or Tg(CMV-CreERT2); Ptpn11^fl/– mice were treated daily with 4-OHT or vehicle control (EtOH, -) and harvested after 1 and 3 weeks. Bottom: pellets from wild-type mice were treated daily with a MEK1/2 inhibitor (U0126), or vehicle control (DMSO, -), and harvested after 1 and 3 weeks. B: Number of transcripts that had significantly lower (blue) or higher (red) abundance in treated pellets compared to vehicle treated pellets, either at w1 or w3. Transcripts that changed in abundance in Ptpn11 cKO pellets were excluded if they exhibited similar changes in wild-type pellets treated with 4-OHT.