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. 2014 May 29;10(5):e1004338. doi: 10.1371/journal.pgen.1004338

Figure 5. Effect of KRAS induction in HKe3 and HKe3 clone 8 cell lines.

Figure 5

Shown are the results from 3 independent experiments. (A) HKe3 have a period of 25.3±0.59 hours (p<0.05, Student's t-test), (B) HKe3 cells treated with mifepristone show a small period increase (26.1±0.68 hours p<0.05, Student's t-test). (C) HKe3 clone8 present a period of 25.1±0.3 hours (p<0.05, Student's t-test). (D) HKe3 clone8 cells treated with mifepristone show a large period increase (37.79±0.96 hours p<0.05, Student's t-test). (E) Western Blot analysis for RAS and phosphorylated ERK in HKe3 and HKe3 clone8 cells following K-Ras induction by mifepristone. Extracts where prepared from untreated cells (−) or 48 hours after addition of mifepristone (+). Levels of RAS and phosphorylated ERK were analysed. GAPDH was used as loading control.