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. 2014 Jun 1;25(11):1769–1781. doi: 10.1091/mbc.E13-05-0292

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© 2014 Giurisato et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Calcineurin inhibition reduces STIM-ORAI puncta formation. Representative images of HeLa cells coexpressing YFP-STIM1 (0.2 μg) and ORAI1-RFP (0.2 μg). Cells were starved overnight and then stimulated without (basal) or with 1 μM Tg (+Tg) and pretreated or not with 10 μM Cyper or CsA. Bar, 5 μM. White squares show enlarged images. Bar, 1 μM. The quantification of puncta, expressed as mean per cell ± SEM, is based on the number of cells indicated in parentheses: basal (20); Tg (25); CsA+Tg (28); Cyper+Tg (26). The number of puncta formed in response to Tg treatment and the effect of inhibition of CN are shown in the graph bars. Significance of values is compared with control Tg-treated cells. *p < 0.01.