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. 2014 Jun 1;25(11):1793–1807. doi: 10.1091/mbc.E13-10-0599

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© 2014 Ruppersburg and Hartzell. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The nimbus is a hub of the microtubule cytoskeleton. (A) The ANO1 (green) nimbus contains both acetylated (blue) and nonacetylated (red) α-tubulin. Top, representative confocal xy-planes of a z-stack of a nimbus that was subjected to deconvolution. Bottom, the z-stack was deconvolved using Huygens Essential software, and isosurfaces were then constructed from the deconvolved image using Imaris software. Top view, from the apical surface of the cell. Side view, from a plane near the apical surface. (B) EB1 (red) is located at the tips of the microtubules (blue) in the nimbus and commingles with ANO1 (green). Top, representative confocal xy-plane images of a z-stack used for deconvolution. Bottom, isosurfaces constructed from deconvolved images. Scale bars, 2.5 μm.