Skip to main content
. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186

Figure 1. Cas9 directs a wide range of indel frequencies depending on sgRNA base composition.

Figure 1

A. (left) Embryo survival at 24–30 hpf compared to indel frequency. 5′GG is black, 5′GA is blue, 5′AG is red. (right) Distribution of sgRNAs by indel frequency. B. G/C content of sgRNAs compared to indel frequency. Means for each category are indicated in red and the mean across all sgRNAs is indicated in blue. C. Heatmap plot showing position-specific effects on indel rates for nucleotide positions 3–21 (21 is the first base of the PAM). Color scale represents the increase in indel frequency of a given sgRNA containing the indicated nucleotide at the specified position. D. sgRNA 5′ dinucleotide pair compared to indel frequency.