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A. Indel frequencies of 5′GG sgRNAs modified with the addition of a 5′adenine. B. Indel frequencies of sgRNAs modified by substituting the 5′ dinucleotide as indicated. C. Indel frequencies of sgRNAs transcribed using either T7 (repeated data from above) or SP6 polymerase. Altered bases to the template are indicated in bold, mismatched bases to the genome are underlined.
