Figure 3. Map of the high-performance amyE integration vectors pXFP_Star.

The pXFP_Star vector set features the transcriptional terminator of the B. subtilis gyrA gene and a small cloning site (SCS) upstream of the LIC site used for ligation-independent cloning of promoter inserts. The grey cross marks the position of the deleted ldh parts. The vector variants differ only with respect to the fluorophore gfpmut3, iyfp or cfp_Bs. The other elements, annotated as amyE′ =  amyE front, ′amyE =  amyE back, denote homologous amyE parts enabling chromosomal integration into the amyE locus. β-lactamase gene (bla) and chloramphenicol acetyltransferase gene (cat) are required for selection in E. coli and B. subtilis respectively. The grey arrow denotes the ColE1 region required for plasmid replication with the ColE1 origin of DNA replication (+1) highlighted in magenta.