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. 2014 May 29;9(5):e98360. doi: 10.1371/journal.pone.0098360

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© 2014 Trauth, Bischofs

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histograms of the fluorescence intensity distribution for the rapE promoter fusion (PrapE) and the “empty” vector reference strain (EV) obtained with the pGFP_Star (top panel) and the pGFPamy (bottom panel) reporter system, respectively. The left column shows the results measured at OD_600nm = 2.5 when the rapE promoter is repressed and the right panel shows results obtained at OD_600nm = 6 when the promoter is weakly active. For the pGFPamy reporter system PrapE cells produce less fluorescence than the EV. Hence, spurious upstream transcription and rapE promoter transcription do not contribute additively to the fluorescence signal but interfere with each other in unpredictable manners. The Star-system is capable to reliably report on rapE promoter activity.