Table 1. Strains and plasmids.
| Strains/Plasmids | Genotype | Source |
| Plasmids | ||
| pGFPamy | ‘amyE cat LIC promoterless gfpmut3 amyE’ bla ColE1 origin | [9] |
| pYFPamy | ‘amyE cat LIC promoterless iyfp amyE’ bla ColE1 origin | [9] |
| pCFPamy | ‘amyE cat LIC promoterless cfpBs amyE’ bla ColE1 origin | [9] |
| pGFPbglS | bglS′ LIC promoterless gfpmut3 nptIII ′bglS bla ColE1 origin f1(+)origin | [9] |
| pGFP_Star | ‘amyE cat TgyrA LIC promoterless gfpmut3 amyE’ bla ColE1 origin | This study |
| pYFP_Star | ‘amyE cat TgyrA LIC promoterless iyfp amyE’ bla ColE1 origin | This study |
| pCFP_Star | ‘amyE cat TgyrA LIC promoterless cfpBs amyE’ bla ColE1 origin | This study |
| pGFP_Star_P rapE | ‘amyE cat TgyrA PrapE-gfpmut3 amyE’ bla ColE1 origin | This study |
| pGFPamy_P rapE | ‘amyE cat PrapE-gfpmut3 amyE’ bla ColE1 origin | This study |
| Strains | ||
| E. coli | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | [35]; Gift of Karl Kochanowski |
| BW25113_pUA139 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 nptII promoterless gfpmut2 sc101 origin | Strain [35]; Plasmid [1]; Gift of Karl Kochanowski |
| B. subtilis | ||
| 168 1A700 | trpC2 | Gift of Oscar Kuipers |
| 168_pGFPamy * | trpC2 amyE:: promoterless gfpmut3 cat | This study |
| 168_pYFPamy * | trpC2 amyE:: promoterless iyfp cat | This study |
| 168_pCFPamy * | trpC2 amyE:: promoterless cfpBs cat | This study |
| 168_pGFP_Star * | trpC2 amyE:: TgyrA promoterless gfpmut3 cat | This study |
| 168_pYFP_Star * | trpC2 amyE:: TgyrA promoterless iyfp cat | This study |
| 168_pCFP_Star * | trpC2 amyE:: TgyrA promoterless cfpBs cat | This study |
| 168_pGFPbglS | trpC2 bglS:: promoterless gfpmut3 nptIII | This study |
| 168_P rapE-gfp (Star) * | trpC2 amyE:: TgyrA PrapE-gfpmut3 cat | This study |
| 168_P rapE-gfp (amy) * | trpC2 amyE:: PrapE-gfpmut3 cat | This study |
* two independent clones are used for this study.