Figure 1. Effect of HDACIs on the ex vivo expansion of CB CD34⁺, CD34⁺CD90⁺, and CD34⁺CD90⁺CD184⁺ cells.

(A) Schematic representation of the ex vivo expansion strategy of primary CB CD34⁺ cells (PCs). Freshly isolated PCs were primed for 16 hours with cytokines either in SF or SC media. Cells were then further treated for 7 days under the mentioned culture conditions with or without additional cytokines and in the presence or absence of HDACIs. The expanded and reisolated CD34⁺ cells were used for further analyses. Individual PCs were treated in the absence (control) or presence of VPA, SCR, or CAY10433 (C433) for 7 days in SF media with cytokines. VPA led to the generation of a significantly greater absolute number of CD34⁺ cells (*P < 0.05; **P < 0.005) (B), CD34⁺CD90⁺ cells (*P < 0.05; **P < 0.005) (C), and CD34⁺CD90⁺CD184⁺ cells (***P = 0.0005) (D) per CB collection (mean ± SEM; ANOVA, P ≤ 0.0007 [B and C] and ANOVA, P < 0.0001 [D]) than did other HDACIs (n = 6–7). C, control.