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. 2014 Apr 24;124(6):2378–2395. doi: 10.1172/JCI70313

Figure 6. Effect of HDACIs on HDAC protein expression levels.

Figure 6

CB-MNCs were freshly isolated and treated in the absence and presence of SCR, C433, or VPA for 24 hours. Total cell lysates were prepared, and Western blotting was performed using HDAC mAbs specific to class I (HDAC1, -2, and -3), class IIa (HDAC4 and -5), and class IIb (HDAC6) HDACs as described in Methods. β-Actin was used as a loading control. One of four representative experiments is shown. Un-, untreated freshly isolated CB-MNCs.