Figure 6. CD8⁺ T cell–intrinsic TGF-β signaling results in poor development of E. cuniculi–specific effector CD8⁺ T cell response in aged mice.

(A) Mixed bone marrow chimera generation using a combination of young or aged WT and young CD4-DNR donors. (B) Percentage of splenic KLRG1⁺ CD8⁺ T cells was evaluated in these chimeras at day 12 after infection. (C–E) Effector CD8⁺ T cell polyfunctionality (IFN-γ, Gzb, and/or TNF-α) was assayed in chimeric mice by polychromatic flow cytometry. Data represent 2 experiments with 3–4 chimeras per group. Numbers in dot plots denote percentages.