Figure 4. Enrichment of Du51-specific Tregs in long-term CD40Ig-treated recipients.

T cell–enriched spleen (A) and total graft (B) from long-surviving recipients or from naive animals were incubated with RT1.A^a/Du51 tetramers labeled with PE- and APC-conjugated streptavidin. Top panels show representative plots. Bottom panels show results for the percentage of Tet⁺ cells among each population (left), the absolute number of cells (middle), and the percentage of total organ cells (right) ± SEM. *P < 0.05; **P < 0.01. n = 4–6.