Figure 3. Liraglutide requires CNS GLP1R for anorectic and weight-lowering effects in high-fat–fed mice.

(A) Longitudinal body weights of Glp1r mutant mice treated with a long-acting GLP1R agonist or saline. (B) Cumulative weight loss of 14 days. (C) Longitudinal 14-day cumulative food intake of mice. (D) Total food intake during last 7 days of treatment. (E) Fourteen-day fat-mass change of mice. (F) Fourteen-day lean-mass change in mice. Black, controls; red, GLP1R KD^ΔNestin; gray, GLP1R KD^ΔPhox2b; solid, saline treatment; patterned, liraglutide treatment. Statistical analysis was with 2-way ANOVA with Tukey’s post-hoc with repeated measures (A and C) or without repeated measures (B and D–F). *P < 0.05 compared with saline treatment, same genotype unless otherwise indicated.