Table 1. List of proteins identified by yeast-two-hybrid analyses using intracellular domains of SmVKR1 and SmVKR2 as baits.
| Functional group | Identified proteins | Accession N° | Identified with | Overexpression in ovary | |||
| SmVKR1 | SmVKR2 | S. mansoni | S. japonicum | ||||
| Cytoskeleton arrangement | Fibrillin 2 | U54588.1 | ✗ | ||||
| Rho GTPase (SmRho1) | * | AY158212.1 | ✗ | ✓ | ✓ | ||
| Spectrin, Beta chain | XM_002579838.1 | ✗ | ✓ | ||||
| Zyxin/trip6 | XM_002575737.1 | ✗ | |||||
| Actin | XM_002580135.1 | ✗ | ✗ | ✓ | ✓ | ||
| Prefoldin subunit | XM_002578485.1 | ✗ | ✗ | ✓ | ✓ | ||
| Membrane trafficking | Junctophilin-like protein | XM_002576132.1 | ✗ | ||||
| Synaptotagmin | XM_002576619.1 | ✗ | ✓ | ✓ | |||
| Coatomer gamma subunit | XM_002580203.1 | ✗ | ✗ | ||||
| Gene expression | Expressed protein (similar to ARID2) | XM_002577690.1 | ✗ | ||||
| Expressed protein (Btz domain) | XM_002572591.1 | ✗ | |||||
| Ftz-F1 interacting protein | AY456264.1 | ✗ | ✓ | ||||
| HRX histone methyltransferase | XM_002574332.1 | ✗ | |||||
| Notch | XM_002574857.1 | ✗ | ✓ | ||||
| Nuclear autoantigenic sperm protein | XM_002577077.1 | ✗ | ✓ | ||||
| Peter-pan related protein | XM_002580320.1 | ✗ | ✓ | ✓ | |||
| Plac8 homolog | XM_002573812.1 | ✗ | ✓ | ||||
| Pre-mRNA processing protein prp39-related | XM_002579875.1 | ✗ | ✓ | ✓ | |||
| TFIIA Large subunit | XM_002579091.1 | ✗ | ✓ | ✓ | |||
| tRNA d(2)-isopentenylpyrophosphatase | XM_002571087.1 | ✗ | |||||
| Cellular nucleic acid binding protein | XM_002581028.1 | ✗ | ✗ | ✓ | |||
| Sirtuin 7 (Sirt7) | * | KC993857.1 | ✗ | ✗ | ✓ | ||
| Phosphosignalling | Mek7 | * | XM_002574592.1 | ✗ | ✓ | ||
| PP2C | * | XM_002575792.1 | ✗ | ✓ | ✓ | ||
| Shb-like protein | * | JN864885.1 | ✗ | ||||
| Cbl | XM_002571919.1 | ✗ | ✓ | ||||
| Protein synthesis | Elongation factor 1-alpha | Y08487.1 | ✗ | ✓ | ✓ | ||
| Methionyl aminopeptidase 2 (M24 family) | XM_002572527.1 | ✗ | ✓ | ||||
| Ribosomal protein S2 | XM_002572774.1 | ✗ | |||||
| 40S ribosomal protein S28, putative | XM_002573998.1 | ✗ | ✓ | ✓ | |||
| PDE-12 like protein | * | XM_002577997.1 | ✗ | ✓ | |||
| 60S ribosomal protein L26-like | DQ480541.1 | ✗ | ✓ | ||||
| Elongation factor Tu GTP binding domain containing 1 isoform 6-related | XM_002571419.1 | ✗ | ✗ | ||||
| Diverse enzymes | ATP-dependent transporter | XM_002576209.1 | ✗ | ✓ | |||
| Autophagy-related protein 101 like | XM_002577493.1 | ✗ | |||||
| Carboxypeptidase | XM_002581470.1 | ✗ | ✓ | ||||
| Cathepsin B | M21309.1 | ✗ | ✓ | ✓ | |||
| Chitobiosyldiphosphodolichol alpha-mannosyltransferase | XM_002571918.1 | ✗ | |||||
| GAPDH | XM_002576948.1 | ✗ | ✓ | ||||
| Glutathione peroxidase | M86510.1 | ✗ | ✓ | ||||
| Microsomal glutathione s-transferase | XM_002576747.1 | ✗ | ✓ | ||||
| NADH-cytochrome B5 reductase | XM_002576573.1 | ✗ | ✓ | ||||
| NADH-ubiquinone oxidoreductase 24 kD subunit | XM_002578142.1 | ✗ | ✓ | ✓ | |||
| Ornithine aminotransferase | EU042598.1 | ✗ | ✓ | ||||
| Peptidylprolylisomerase | XM_002577985.1 | ✗ | |||||
| Subfamily T1A non-peptidase homologue (T01 family) | XM_002574652.1 | ✗ | ✓ | ||||
| Thimet oligopeptidase (M03 family) | XM_002574355.1 | ✗ | ✓ | ||||
| Zinc metalloproteinase | XM_002573446.1 | ✗ | |||||
| 26S protease regulatory subunit S10b | XM_002573132.1 | ✗ | |||||
| Endoglycosylceramidase | XP_002577487.1 | ✗ | |||||
Proteins were classified in functional groups and Genbank accession numbers are indicated. Ticks indicate sequences already shown to be overexpressed in ovary by transcriptomic analyses of S. mansoni [69] and S. japonicum [70]. Asterisks indicate the proteins for which the interaction of full length sequences with VKR has been confirmed in yeast.