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. 2014 May 29;10(5):e1004166. doi: 10.1371/journal.ppat.1004166

Figure 5. K22 affects formation of coronavirus replication complex in cells.

Figure 5

MRC-5 cells were infected with wild type HCoV-229E (WT) and K22-resistant recombinants HCoV-229EH121L (H121L), HCoV-229EM159V (M159V), and HCoV-229EH121L/M159V (H121L/M159V) and incubated for 18 h with or without the presence of K22. The cells were then fixed with 4% paraformaldehyde and immunostained for immunofluorescence analysis. Note the lack of detection of dsRNA and nsp8 upon K22 treatment (4 µM) of cells infected with WT but not recombinant viruses. Scale bar is 10 µM.