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. 2014 May 29;10(5):e1004171. doi: 10.1371/journal.ppat.1004171

Figure 5. KF116 impairs formation of dense cores in HIV-1 virions.

Figure 5

(A) Representative thin-section electron micrographs of HIV-1 virions produced in the presence of DMSO or 1 µM KF116. (B) Quantitative analysis of mature virions prepared in the presence of DMSO or 1 µM KF116. Correctly matured electron dense cores are shown in black and eccentric virions lacking electron density are shown in gray. Standard errors determined from two independent experiments are shown. Images of at least 50 mature virions were examined from each experiment. (C) Sucrose density gradient fractionation of detergent-lysed HIV-1 virions produced in HEK293T cells in the presence of DMSO or 1 µM KF116. Cell-free virions were harvested, detergent-lysed, and separated on 30–70% linear sucrose density gradients. Twenty-one 0.5 ml fractions were collected from the top of the gradient and subjected to SDS-PAGE and immunoblotted with HIV-1 Gag antisera to monitor the distribution of HIV-1 capsid. Positions of Gag p24 (capsid) and Gag p17 (matrix) are indicated. (D) Quantitation of HIV-1 capsid (p24) signal intensity from (B) as measured by ImageJ software. Graph represents the relative distribution of HIV-1 capsid (p24) in each of the sucrose density gradient fractions.