FIG. 4.

Western blot analysis of mitotic spindle assembly checkpoint protein. Western blot analysis of BUBR1 and phosphorylated BUBR1 in control lysates treated with 100nM paclitaxel 16 h. Cells were treated with their respective IC₅₀ cisplatin (CP) (A2780, 4 μM; A2780/CP70, 40 μM) or CP plus 20 μM sodium arsenite (CPA) at 37 or 39°C (hyperthermia) for 1 h. Treated cells were washed with PBS and refed fresh media and incubated at 37°C. Protein lysates were prepared at 36 h after treatment for Western blot analysis. Cell treated with 100nM paclitaxel (Pax) as positive control. Data are representative of triplicate biological experiments. GAPDH served as loading control.