FIG. 5.

Western blot analysis of protein markers of G1 arrest. A. Western blot analysis of CDKN1A and pRB Ser807/811P. Panel (a) is CP 37°C, (b) is CP 39°C, (c) is CPA 37°C, and (d) is CPA 39°C. B. Western blot analysis of cyclin E. Cells were treated with their respective IC₅₀ cisplatin (CP; A2780, 4 μM; CP70, 40 μM) or CP plus 20 μM sodium arsenite (CPA) at 37 or 39°C (hyperthermia) for 1 h. Cells were washed with PBS and refed with fresh media and incubated at 37°C. Cell lysates were prepared at 0, 6, 12, 24, and 36 h. ß-actin and GAPDH are loading controls. A2780 and A2780/CP70 cells were treated with 100nM paclitaxel (Pax) for 16 h and served as negative control for cyclin E. Data are representative of triplicate biological experiments.