FIG. 6.

Apoptotic cell death determination using FITC Annexin V propidium iodide assay. Cells were treated with their respective IC₅₀ cisplatin (CP) (A2780, 4 μM; CP70, 40 μM), or CP plus 20 μM sodium arsenite (CPA) at 37 or 39°C (hyperthermia) for 1 h. Treated cells were washed with PBS and refed fresh media and incubated at 37°C for 36 h. Cell death was determined at 36 h after treatment. Data are means ± SD of triplicate biological experiments. Statistical analysis was performed using one-way ANOVA and Tukey test. p < 0.05, a = compared with untreated (UT) A2780 cells, b = compared with untreated A2780/CP70 cells.