Figure 4.

SA, JA, JA-Ile, and IAA profiling upon P. syringae and P. cucumerina infection. Plants were challenged as described in Figure 1. Both mock and pathogen infected plants were harvested at different time-points. Freeze dried material was processed for a targeted quantification analysis by TQD-MS. The concentration of the hormones was determined in all the samples by normalizing the chromatographic area for each compound with the dry weight of the corresponding sample. Leaf material from 15 individual plants for P. syringae (A) resistance assays and 150 plants for P. cucumerina (B) resistance assays were pooled together for each treatment × genotype combination. Data represent average three independent experiments ±SD; n = 3.