Figure 3.
(Left) SAXS simulated curves obtained at increasing BLG concentration in solution (from bottom to top, open squares, circles, up-triangles, down-triangles and diamonds correspond to 2, 4, 6, 8 and 10 g l−1, respectively) and their best fits obtained with GENFIT (solid red lines). All SAXS data were simulated at ambient pressure and temperature, at pH 2.3, and at 100 mM ionic strength. The structures of the BLG monomer and dimer are depicted using the Rasmol software (Bernstein et al., 2000 ▶). The best fit values of the dissociation free energy and the relative mass density of the hydration shell are ΔG dis/(k B T) = 8.22 ± 0.08 and 1.08 ± 0.01, respectively. (Right) BLG monomer fraction in solution versus BLG concentration as obtained from the dissociation free energy.