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. 2014 May 30;4:5125. doi: 10.1038/srep05125

Figure 3. Differentiation of primary mouse bone marrow cells.

Figure 3

(a), optical observation of colony formation of cells cultured on the assemblies. Magnification: ×5. (b), ALP staining of cells cultured on the different substrates. From the left to the right: commerical culturing plate, bare glass plate, the naturally-dried aggregates, the stripe-like assemblies fabricated by 20 mT field, the stripe-like assemblies fabricated by 40 mT field, the stripe-like assemblies fabricated by 80 mT field, the stripe-like assemblies fabricated by 120 mT field. (c) and (d), mRNA expression of ALP/Osteocalcin and BMP-2/RUNX2 of cells on different substrates, respectively. Here Cur-plate means the commercial culturing plate for cells. N-glass means the bare glass plate. Nat-agg means the naturally-dried aggregates of nanoparticles. Ass-20, Ass-40, Ass-80 and Ass-120 mean the stripe-like assemblies fabricated under the 20 mT, 40 mT, 80 mT and 120 mT field strength, respectively. *p<0.05 (calculated between two samples) (e), Western Blotting measurement of ALP expression. From the left to the right: commerical culturing plate, bare glass plate, the naturally-dried aggregates, the stripe-like assemblies fabricated by 20 mT field, the stripe-like assemblies fabricated by 40 mT field, the stripe-like assemblies fabricated by 80 mT field, the stripe-like assemblies fabricated by 120 mT field. (f), ALP staining of cells cultured on the different substrates after thermal treatment for over 8 hours. From the left to the right: commerical culturing plate, bare glass plate, the naturally-dried aggregates, the stripe-like assemblies fabricated by 20 mT field, the stripe-like assemblies fabricated by 40 mT field, the stripe-like assemblies fabricated by 80 mT field, the stripe-like assemblies fabricated by 120 mT field. (g), ALP staining of cells cultured on the different substrates in the presence of magnetic field. From the left to the right: commerical culturing plate, bare glass plate, the naturally-dried aggregates, the stripe-like assemblies fabricated by 20 mT field, the stripe-like assemblies fabricated by 40 mT field, the stripe-like assemblies fabricated by 80 mT field, the stripe-like assemblies fabricated by 120 mT field.