Table 1.
Sequence of primers used in this study
| Gene Name | Primer designation | Primer sequence |
|---|---|---|
| I-SceI Flank | Flank_Fw | GAG CAG GAA CCT GAG GAG |
| Flank_Rv | GCC GTA GGT ATT ACC CTG | |
| I-SceI 250bp | 250bp_Fw | CAT GCC CGA AGG CTA CGT |
| 250bp_Rv | CGG CGC GGG TCT TGT A | |
| I-SceI 300bp | 300bp_Fw | CTT CTT CAA GGA CGA CGG C |
| 300bp_Rv | GTA GTT GTA CTC CAG CTT GTG | |
| I-SceI 350bp | 350bp_Fw | CTA CAG CTC CTG GGC AAC |
| 350bp_Rv | CTT CGG CAC CTT TCT CTT C | |
| I-SceI 2200bp | 2200bp_Fw | CGC GAC GTC TGT CGA GAA G |
| 2200bp_Rv | GCC GAT GCA AAG TGC CGA TA | |
| GAPDH | GAPDH_Fw | GAA GGT GGA GGT CGG AGT C |
| GAPDH_Rv | GAA GAT GGT GAT GGG ATT TC |