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. 2014 May 30;4:5074. doi: 10.1038/srep05074

Figure 1. Detection of VSV DNA in 293T human cells.

Figure 1

(a) DNA was extracted from 293T cells at 0, 1, 2, 4, 6, or 16 hr after infection with VSV at an MOI of 0.1 and subjected to PCR amplification for the VSV N gene using the N478-F/N681-R primer pair. The VSV inoculum (Sup) (lane 7) and mock-infected samples (lane 8) were included as controls. The samples prepared 1 hr after inoculation (lane 2) or VSV Sup did not produce VSV DNA. The DNA sequence of the PCR product obtained from the cells harvested at 16 hr post-infection (lane 6) was verified to be the expected VSV N amplicon. (b) DNA samples were harvested at 16 hr post-infection and were treated with RNase A or DNase I and subsequently used for PCR. The VSV DNA was not detected after DNase I treatment (lane 2). All samples used in Figures 1a and 1b were prepared in a single experiment, and cropped gel images are shown.