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. 2014 May 30;4:5074. doi: 10.1038/srep05074

Figure 8. Synthesis of complementary DNA to RNA of echovirus type 30 and RSV in human cells.

Figure 8

(a) The DNA was extracted from RD cells 48 hr after infection with echovirus type 30 and subjected to PCR with the echovirus type 30-specific primers. (b) The RSV DNA was detected in HEp-2 cell lysates prepared five days post-infection with RSV. The DNA sequences of these bands were determined after cloning and found to be identical to the sequences of the viral strains used (GenBank: echovirus type 30, AF311938; RSV, AY911262). Viral RNAs were extracted from echovirus type 30 and RSV propagated in RD and Hep-2 cells, respectively. cDNAs to these RNAs were made, purified and used as templates for PCR (lane 1). RD and HEp-2 cells were infected with echovirus type 30 and RSV, incubated and lysed as described in the Methods section. The cell lysates were used for PCR (lane2). The lysates of mock-infected RD and Hep-2 cells were used as controls (lane 3). Note that cropped gel images are shown and full-length gel images are presented in the supplementary information.