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. Author manuscript; available in PMC: 2014 May 30.
Published in final edited form as: J Neural Eng. 2013 Jul 17;10(4):046017. doi: 10.1088/1741-2560/10/4/046017

Figure 1.

Figure 1

Simultaneous multiphoton calcium imaging with electrophysiology. (a) Maximum intensity projection showing juxtacellular recording from a Purkinje cell dendrite. The tissue was loaded with Oregon Green BAPTA-1 AM calcium indicator dye (green), and the pipette filled with Alex 594 (red) to aid visualization during targeted recording. (b) Imaged location, corresponding to grey horizontal line in (a). (c) Mask showing region of interest for the recorded Purkinje cell. (d) Simultaneous acquisition of fluorescence time series (shown unfiltered) and dendritically recorded complex spikes (CS), showing CS-driven calcium transients.