Fig. 8.

Induction of centrosome disjunction by C-Nap1 hyperphosphorylation. The left-hand side of this schematic model summarizes our current understanding that Mst2-driven recruitment of Nek2, together with inactivation of PP1 and potentially phosphorylation by other kinases, leads to C-Nap1 hyperphosphorylation at the G2/M transition. This disturbs the interaction of C-Nap1 with Cep135, with C-Nap1 itself and possibly with rootletin, leading to disassembly of the intercentriolar linker and driving centrosome disjunction. On the right-hand side, we hypothesize that Sas-6 and C-Nap1 might compete for binding to Cep135, and that degradation of Sas-6 at the end of mitosis would enable C-Nap1 recruitment and re-establishment of the linker as cells pass from mitosis back into G1. Blue, centrioles.