Table 1.
Primary antibodies used in this study.
| Antibody | Type | Specificity | Phosphorylation sites | Source/reference |
|---|---|---|---|---|
| IRβ | Poly- | IRβ | Cell Signaling Technology, Danvers, MA | |
| IGF-1Rβ | Poly- | IGF-1Rβ | Cell Signaling Technology | |
| P-IRβ/IGF-1Rβ | Mono- | P-IRβ/IGF-1Rβ | Tyr1150/1151(IRβ), Tyr1135/1136 (IGF-1Rβ) | Cell Signaling Technology |
| IRS1 | Poly- | IRS1 | Cell Signaling Technology | |
| IRS1 pS307 | Poly- | P-IRS1 | Ser307 | Cell Signaling Technology |
| PI3K p85 | Poly- | PI3K (p85) | Cell Signaling Technology | |
| P-PI3K p85 | Poly- | P-PI3K (p85) | Tyr458/Tyr199 | Cell Signaling Technology |
| PDK1 | Poly- | PDK1 | Cell Signaling Technology | |
| PDK1 pS241 | Poly- | P-PDK1 | Ser241 | Cell Signaling Technology |
| AKT | Poly- | AKT | Cell Signaling Technology | |
| AKT pS473 | Poly- | P-AKT | Ser473 | Cell Signaling Technology |
| AKT pT308 | Poly- | P-AKT | Thr308 | Cell Signaling Technology |
| GSK-3β pS9 | Poly- | P-GSK-β | Ser9 | Cell Signaling Technology |
| GSK-3α/β | Poly- | GSK-3β | Cell Signaling Technology | |
| R134d | Poly- | Tau | Tatebayashi et al. (1999) | |
| pS199 | Poly- | P-tau | Ser199 | Invitrogen, Grand Island, NY |
| pT205 | Poly- | P-tau | Thr205 | Invitrogen |
| pT212 | Poly- | P-tau | Thr212 | Invitrogen |
| pS214 | Poly- | P-tau | Ser214 | Invitrogen |
| pT231 | Poly- | P-tau | Thr231 | Invitrogen |
| pS262 | Poly- | P-tau | Ser262 | Invitrogen |
| 12E8 | Mono- | P-tau | Ser262/356 | Dr. D. Schenk |
| PHF-1 | Mono- | P-tau | Ser396/404 | Dr. P. Davies |
| pS409 | Poly- | P-tau | Ser409 | Invitrogen |
| pS422 (R145) | Poly- | P-tau | Ser422 | Pei et al. (1998) |
| CaMKII | Mono- | CaMKII | Promega, Madison, WI | |
| CaMKII(pT286) | Poly- | P-CaMKII | Thr286 | Promega |
| JNK | Poly- | JNK | Cell Signaling Technology | |
| P-JNK | Poly- | P-JNK | Thr183/Tyr185 | Cell Signaling Technology |
| ERK1/2 | Mono- | ERK1/2 | Cell Signaling Technology | |
| P-ERK1/2 | Poly- | P-ERK1/2 | Cell Signaling Technology | |
| CDK5 | Poly- | CDK5 | Santa Cruz Biotechnology | |
| P35 | Poly- | P35 | Santa Cruz Biotechnology | |
| PP2A-C | Mono- | PP2A-C | BD Bioscience, Palo Alto, CA | |
| Methyl-PP2A-C | Mono- | Methyl-PP2A-C | Millipore, Temecula, CA | |
| PP2A pY307 | Poly- | P-PP2A | Tyr307 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Anti-GAPDH | Poly- | GAPDH | Santa Cruz Biotechnology |